团队擅长药物的临床前评价，主要是以下方面：

（1）构建神经精神疾病动物模型并用动物模型进行临床前药物筛选

认知损害建模及检测： 空间记忆、 恐惧记忆、 工作记忆、 新异物体识别记忆

脑中风：局灶性短暂及永久性模型

精神分裂症：啮齿类动物构建精分模型

药物成瘾：自身给药、颅内自身电刺激及条件位置偏好

焦虑：高架十字迷宫

抑郁：强迫游泳、悬尾及糖水偏好

脊髓损伤：挫伤及机械损伤

在体行为药理模型以及微透析、颅内显微注射、静脉自身给药、

（2） 离体细胞模型进行药物的临床前评价

氧糖剥夺，离体血脑屏障模型建立，原代神经元培养，原代少突胶质细胞

1、GGF2通过miR-17~92/Pdlim5/ID2信号通路促进NG2细胞增殖分化改善卒中后神经功能的作用及机制研究，国家自然科学基金面上项目；

2、慢性尼古丁暴露调控miR-21/Pdlim5减轻急性脑缺血血脑屏障损伤及其分子机制研究，国家自然科学基金面上项目；

3、多巴胺受体偶联的信号通路介导急性脑缺血溶栓期内血脑屏障损伤的机制研究（结题）；江苏省自然科学基金面上项目。

1. 急性脑梗后如何减少tPA溶栓后的脑出血？

吸烟病人溶栓后出血率比正常人低，从这个现象出发提出尼古丁可减轻脑缺血及tPA引起的血脑屏障损伤

2. 如何促发脑梗后的神经功能恢复？

从白质和血脑屏障完整性出发，刺激内源性干细胞的增值和分化来促进中风后的神经功能恢复

3. 如何减轻精神分裂症引起的认知损害？

精神分裂症病人的吸烟率高，从这个现象提出尼古丁减轻精神分裂症引起的认知损伤

发表论文：

1. Xiaona Wang, Gai-Xiu Xue, Wen-Cao Liu, Hui Shu, Mengwei Wang, Yanyun Sun, Xiaojing Li, Yi Sun, Chun-Feng Liu, Jie LiuCA, Wenlan LiuCA, Xinchun JinCA. Melatonin alleviates lipopolysaccharide-compromised integrity of blood brain barrier by activation of AMP-activated protein kinase (AMPK) in old mice. Aging cell  2017, 16(2):414-421   IF 7.6  引用20次（5 Year Impact Factor:9.951）

2. Yushan Liu, Wencao Liu, Yanyun Sun, Xianzhi Shen, Xiaona Wang, Rong Pan, Hui Shu, Chun-Feng Liu, Wenlan Liu, Ke jian Liu, Xinchun JinCA. Normobaric hyperoxia extends neuro- and vaso-protection of N-Acetylcysteine in transient focal ischemia. Molecular Neurobiology 2017, 54:3418–3427  IF 6.1 引用14 次    2017 年中国脑卒中防治百篇优秀论文“北斗奖” （5 Year Impact Factor:5.512）

3. Shuang Chen#, Yanyun Sun#, Fei Li#, Xinyu Zhang, Wenlan Liu, Guo-qing ZhengCA, Xinchun JinCA. Modulation of α7nAchR by melatonin alleviates ischemia and reperfusion-compromised integrity of blood brain barrier through inhibiting HMGB1-mediated microglia activation and CRTC1-mediated neuronal loss. Cellular and  Molecular Neurobiology, 2021 In Press IF 5.046

4. Yan Wang#, Xiaona Wang#, Xinyu Zhang#, Shuang Chen#,Yanyun Sun, Wenlan Liu, Xinchun JinCA , Guo-qing ZhengCA. D1 receptor-mediated endogenous tPA upregulation contributes to blood brain barrier damage after acute ischemic stroke. Journal of Cellular and Molecular Medicine, 2020, 24(16):9255-66.  IF 5.3（5 Year Impact Factor:5.533）

5. Hui Shu#, Mengwei Wang#, Min Song#, Yanyun Sun#, Xianzhi Shen, Junfang Zhang, and Xinchun JinCA. Acute nicotine treatment alleviates LPS-induced impairment of fear memory reconsolidation through AMPK activation and CRTC1 upregulation in hippocampus. 2020 International Journal of Neuropsychopharmacology. 2020, 23(10):687-699. IF 5.07（5 Year Impact Factor:5.168）

6. Fei Li#, Wen-Cao Liu#, Qi Wang, Yanyun SunCA, Hongbo WangCA, Xinchun JinCA. NG2-glia cell proliferation and differentiation by glial growth factor 2 (GGF2), a strategy to promote functional recovery after ischemic stroke. Biochemical Pharmacology, in Press 2020. IF 5.858（5 Year Impact Factor:5.675）

7. Xinyu Zhang#, Xianzhi Shen#, Jiali Dong#, Wen-Cao Liu#, Yanyun Sun, Hui Shu, Wenlan LiuCA, Chun-feng LiuCA, Xinchun JinCA Inhibition of reactive astrocytes with fluorocitrate alleviates ischemic stroke-induced memory impairment through upregulating CRTC1 and synaptophysin in rat. Cellular and  Molecular Neurobiology    2019, 39:1151–1163.   IF   3.89（5 Year Impact Factor:4.780）

8. Yanyun Sun, Xi Chen, Xinyu Zhang, Xianzhi Shen, Mengwei Wang, Xiaona Wang, Wen-Cao Liu, Chun-Feng Liu, Jie Liu,Wenlan Liu, Xinchun JinCA. ß2-adrenergic receptor mediated HIF-1a upregulation during acute cerebral ischemia. Frontiers in Molecular Neuroscience  2017, 10:257  IF   5.07  引用16次       获得苏州市自然科学学术论文优秀奖（5 Year Impact Factor:5.760）

9. Hui Shu, Guo-qing Zheng, Xiaona Wang, Yanyun Sun, Yushan Liu, John Weaver, Xianzhi Shen, Wenlan LiuCA, Xinchun JinCA. Activation of matrix metalloproteinase in dorsal hippocampus drives improvement in spatial working memory after intra-VTA nicotine infusion in rats. Journal of Neurochemistry 2015, 135(2):357-367.  IF 4.28引用6 次（5 Year Impact Factor:5.690）

10. Xianzhi Shen, Yanyun Sun, Mengwei Wang, Hui Shu, Junfang ZhangCA, Xinchun JinCA. Chronic N-acetylcysteine treatment alleviates lipopolysaccharide-induced working memory deficit through upregulating caveolin-1 and synaptophysin in mice. Psychopharmacology 2018, 235(1):179-191 IF 3.54  引用7次（5 Year Impact Factor:4.077）

11. Yufei Shen, Jingxia Gu, Ziyun Liu, Congying Xu, Shuxia Qian, Xiaoling Zhang, Beiqun Zhou, Qiaobing Guan, Yanping WangCA, Xinchun JinCA. Inhibition of HIF-1α reduced blood brain barrier damage by regulating MMP-2 and VEGF during acute cerebral ischemia. Frontiers in Cellular Neuroscience  2018 12:200   IF  4.3引用7 次2017 年中国脑卒中防治百篇优秀论文“思南奖”（5 Year Impact Factor:5.574）

12. Yuan Zhang, Ke Yang, Ting Wang, Weiping Li, Xinchun JinCA,Wenlan LiuCA. Nrdp1 increases ischemia induced primary rat cerebral cortical neurons and pheochromocytoma cells apoptosis via downregulation of HIF-1α protein. Frontiers in Cellular Neuroscience   2017, 11:293  IF  4.55引用3次（5 Year Impact Factor:5.574）

13. Wen-Cao Liu, Xiaona Wang, Xinyu Zhang, Xi Chen, Xinchun JinCA. Melatonin supplementation, a strategy to prevent neurological diseases through maintaining integrity of blood brain barrier in old people. Frontiers in Aging Neuroscience 2017, 9:165   IF 4.5  引用8次 （5 Year Impact Factor:5.913）

14. Xiaona Wang, Yushan Liu, Yanyun Sun,Wenlan Liu, Xinchun JinCA. Blood brain barrier breakdown was found in non-infarcted area after 2-h MCAO. Journal of Neurological Sciences 2016, 363:63-68.    IF 2.44   引用5 次（5 Year Impact Factor:2.661）

15. Xinchun JinCA, Yanyun Sun, Ji Xu, Wenlan Liu. Caveolin-1 mediates tissue plasminogen activator-induced MMP-9 upregulation in cultured brain microvascular endothelial cells. Journal of Neurochemistry. 2015,132(6):724-730.   IF 4.28  引用21 次（5 Year Impact Factor:5.690）

16. Jie Liu*, Xinchun Jin*, Ke J Liu, Wenlan Liu. Matrix metalloproteinase-2-mediated occludin degradation and caveolin-1-mediated claudin-5 redistribution contribute to blood-brain barrier damage in early ischemic stroke stage. Journal of Neuroscience 2012, 32(9):3044-57. (共同第一作者)    IF 6.908  引用288次（5 Year Impact Factor:2.661）

17. Xinchun Jin, Jie Liu, Ke J Liu, Yi Yang, Wenlan Liu. Spatiotemporal evolution of BBB damage and tissue infarction within the first 3 hours after ischemia onset. Neurobiology of Disease 2012, 48(3):309-316.  IF 5.624引用 41次（5 Year Impact Factor:6.170）

18. Xinchun Jin, Jie Liu, Ke J Liu, Gary Rosenberg, Yi Yang, Wenlan Liu. Normobaric hyperoxia combined with minocycline provides greater neuroprotection than either alone in transient focal cerebral ischemia. Experimental Neurology 2013, 240C:9-16.   IF 4.645引40次（5 Year Impact Factor:5.340）

19. Xinchun Jin, Svetlana Semenova, Li Yang, Robert Ardecky,  and Athina Markou. The mGluR2 positive allosteric modulator BINA decreases cocaine self-administration and cue-induced cocaine-seeking and counteracts cocaine-induced enhancement of brain reward function in rats. Neuropsychopharmacology 2010, 35, 2021–2036. (亮点介绍. Neuropsychopharmacology 35, 2007–2008).   IF 8.678  引用58次（5 Year Impact Factor:8.210）

20. Svetlana Semenova#, Xinchun Jin#, Tristan McClure-Begley, Matthew Tadman, Michael Marks and Athina Markou. Differential effects of intermittent and continuous nicotine exposure on affective and somatic aspects of nicotine withdrawal and expression of α4β2* nAChRs in rats. Pharmacology Biochemistry Behavior (共同第一作者)  2018,171, 54-65  IF  2.9引用2次

21. Xinchun Jin, Jie Liu and Wenlan Liu. Early ischemic blood brain barrier damage: a potential indicator for hemorrhagic transformation following tissue plasminogen activator (tPA) thrombolysis?  Current Neurovascular Research 2014,11(3):254-262.   IF 2.804 引用32 次（5 Year Impact Factor:2.481）

22. Wen-Cao Liu, Xinchun JinCA. Oxygen or cooling, to make a decision after acute ischemia stroke Medical Gas Research 2016, 6(4):206-211.  引用7 次

23. Lijun Liang, Jinming Yang, Xinchun JinCA. Cocktail treatment, a promising strategy to treat acute cerebral ischemic stroke? Medical Gas Research 2016,6(1), 33-38.  引用13 次

24. Xinchun Jin, Yufei Lu, Xiaofei Yang, Lan Ma, Baoming Li. Glucocorticoid receptor in the basolateral nucleus of amygdala is required for post-reactivation reconsolidation of auditory fear memory. European Journal of Neuroscience 2007, 25, 3702–3712.  IF 3.879  引用74次（5 Year Impact Factor:3.374）

25. Xinchun Jin, Xuelian Qi, Xiaofei Yang, Baoming Li. Protein synthesis inhibition in the basolateral nucleus of amygdala facilitates extinction of auditory fear memory. Chinese Science Bulletin, 52, 2532-2542, 2007.   IF 1.6（5 Year Impact Factor:1.738）

26. Xinchun Jin, Chaolin Ma and Baoming Li. The alpha (2A)-adrenoceptor agonist guanfacine improves spatial learning but not fear conditioning in rats. Acta Physiologica Sinica, 59(6):739-744, 2007. 引用11次

27. Jie Liu, John Weaver, Xinchun Jin, Yuan Zhang, Ji Xu, Ke J Liu, Weiping Li, Wenlan Liu. The interactions between nitric oxide, caveolin-1 and autophagosome contribute to oxygen-glucose deprivation-induced of claudin-5 degradation in endothelial cells. Molecular Neurobiology 53(9):5935-5947, 2016  IF 6.1引用32 次（5 Year Impact Factor:5.512）

28. Peiyun Yan, Zhancheng Xue, Dezhu Li, Saiqi Ni, Chuang Wang, Xinchun Jin, Dongsheng Zhou, Xingxing Li, Xin Zhao, Xiaowei Chen, Wei Cui, Dingli Xu, Wenhua Zhou, Junfang Zhang. Dysregulated CRTC1-BDNF signaling pathway in the hippocampus contributes to Aβ oligomer-induced long-term synaptic plasticity and memory impairment. Exp Neurol.2021

29. Xiaoguang Zhang, Ye Song, Chang Shan, Xi-fan Wu, Yan-hua Tong, Xinchun Jin, Wenlan Liu, Guoqing Zheng, Jie Liu. Borneol attenuates ultrasound-targeted microbubble destruction-induced blood-brain barrier opening in focal cerebral ischemia. Frontiers in Neurology, 2017,8:704   IF 3.55

30. Wen-Cao Liu, Qi Wang, Xinchun JinCA. CRTC1, a target to delay aging-induced cognitive deficit through protecting blood brain barrier. In preparation. 

31. Mengwei Wang, Yanyun Sun, Hui Shu, Xianzhi Shen, Junfang Zhang, Hongqiang Cheng, Xinchun JinCA. Chronic nicotine treatment alleviates schizophrenia-induced memory deficit through upregulating Pdlim5 and CRTC1 in mice. In preparation.

专利：

药物组合物及其应用  2016.5.9-2036.5.8

授权号 ZL201610301881.X