研究组计划逐步发展并完善，动物行为，环路结构，细胞功能及组学特征相结合的神经环路多层次解析技术。以转基因动物、类脑器官为研究模型；整合单细胞测序、特异性标记以及光遗传行为学、在体微镜/双光子成像、多通道膜片钳等多种功能检测技术；对动物特定认知行为，从脑核团及投射关系、局部神经环路连接样式，细胞类别构成、形态及生理特性、以及组学特征等多个层次，开展神经机制解析。在神经功能结构与分子特征间建立联系，发现并验证新的分子靶点，为损伤或疾病状态下神经系统的功能修复，提供分子细胞水平的机制线索和方案。

1. 国家自然科学基金面上项目，32171021，丘脑连接核与前额叶间细胞类别特异性神经环路对恐惧记忆的调控研究，2022/01-2025/12，在研，主持。

2. 北京市自然科学基金面上项目，5222002，小鼠前额叶皮层中细胞类别特异性神经活动调控社交行为的研究，2022/01-2024/12，在研，主持。

3. 科技部国家重点研发计划“干细胞及转化研究” 重点专项，2020YFA0112500，干细胞模拟胚胎发育和器官发生的机制研究及其转化应用，2021/01-2025/12，在研，骨干参与。

4. 科技部国家重点研发计划“发育编程及其代谢调节”重点专项，2020YFA0804000，儿童神经发育异常的遗传调控研究，2020/12-2025/11，在研，骨干参与。

2017-2022年度发表论文17篇，其中并列一作及并列通讯7篇(#, 共同第一作者; *, 共同通讯作者):

1.     ZhongS#, Ding W#, Sun L#, Lu Y#,Dong H, Fan X, Liu Z, Chen R, Zhang S, Ma Q, Tang F, Wu Q*, Wang X*. 2020. Decoding the development of thehuman hippocampus. Nature 577:531-536.

2.     Sun L#,Chen R#, Li L#, Yuan B#, Song K, Pan NC, Cheng T, Chang S, Lin K, He X, Wu Q,Xu F, Qiu Z*, Wang X*. 2020. Visualizationand correction of social abnormalities-associated neural ensembles in adultMECP2 duplication mice. Science Bulletin. 65(14):1192-1202.

3.     ShiY#, Sun L#, Wang M#, Liu J#,Zhong S, Li R, Li P, Guo L, Fang A, Chen R, Ge W, Wu Q*, Wang X*. 2020.Vascularized human cortical organoids (vOrganoids) model cortical developmentin vivo. PLOS Biology 18(5):e3000705.

4.     LiuJ#, Wang M#, Sun L#, Pan NC#,Zhang C, Zhang J, Zuo Z, He S, Wu Q*, Wang X*. 2020. Integrative analysis of in vivo recording with single-cellRNA-seq data reveals molecular properties of light-sensitive neurons in mouseV1. ProteinCell. 11(6):417-432.

5.     Fan X#,Fu Y#, Zhou X#, Sun L#, Yang M, Wang M, Chen R, Wu Q, Yong Y,Dong J, Wen L, Qiao J*, Wang X*, Tang F*. 2020. Single-celltranscriptome analysis reveals cell lineage specification in temporal-spatialpatterns in human cortical development. Science Advance. 6(34):eaaz2978.

6.     Sun L#,*,Chen R#, Bai Y, Li J, Wu Q*, Shen Q*, Wang X*. 2019. Morphological and Physiological Characteristics ofEbf2-EGFP-Expressing Cajal-Retzius Cells in Developing Mouse Neocortex. CerebCortex 29:3864-3878.

7.     Li R#,Sun L#, Fang A#, Li P, Wu Q*,Wang X*. 2017. Recapitulatingcortical development with organoid culture in vitro and modeling abnormalspindle-like (ASPM related primary) microcephaly disease. Protein Cell 8(11):823-833.

8.   Shi Y#,Wang M#, Mi D#, Lu T, Wang B, Dong H, Zhong S, Chen Y, Sun L,Zhou X, Ma Q, Liu Z, Wang W, Zhang J, Wu Q*, Marín O*, Wang X*. 2021. Mouseand human share conserved transcriptional programs for interneuron development.Science. 374(6573):eabj6641.

9.   Xie Z#,Wang M#, Liu Z#, Shang C#, Zhang C, Sun L, Gu H, Ran G, Pei Q, MaQ, Huang M, Zhang J, Lin R, Zhou Y, Zhang J, Zhao M, Luo M, Wu Q*, Cao P*, WangX*. 2021. Transcriptomic encoding of sensorimotor transformation in themidbrain. Elife. 10:e69825.

10.   ZhouX., Liu J, Peng H, Zhong S, Ding W, Sun L, Ma Q, Liu Z, Chen R,Wu Q, Wang X*. 2020. Cellular and molecular properties of neuralprogenitors in the developing mammalian hypothalamus. Nature Communications13;11(1):4063.

11.   Yi W#, Lu Y#, Zhong S#, ZhangM#,*, Sun L, Dong H, Wang M, Wei M, Xie H, Qu H, Peng R, Hong J,Yao Z, Tong Y, Wang W, Ma Q, Liu Z, Ma Y, Li S, Yin C, Liu J, Ma C, Wang X*, WuQ*, Xue T*. 2020. A single-cell transcriptome atlas of the aging humanand macaque retina. National Science Review. nwaa179.

12.   DingW#, Wu Q#, Sun L, Pan NC,Wang X*. 2019. Cenpj Regulates CiliaDisassembly and Neurogenesis in the Developing Mouse Cortex. TheJournal of neuroscience : the official journal of the Society for Neuroscience39:1994-2010.

13.   PanNC#, Fang A#, Shen C, Sun L,Wu Q, Wang X*. 2019. EarlyExcitatory Activity-Dependent Maturation of Somatostatin Interneurons inCortical Layer 2/3 of Mice. Cereb Cortex 29:4107-4118.

14.   FanX#, Dong J#, Zhong S#, Wei Y#, Wu Q, Yan L, Yong J, Sun L, Wang X, Zhao Y, Wang W, Yan J, Wang X*, Qiao J*, TangF*. 2018. Spatial transcriptomicsurvey of human embryonic cerebral cortex by single-cell RNA-seq analysis. CellRes 28:730-745.

15.   ShiY#, Li S#, Wu Q#, Sun L,Zhang J, Pan N, Wang Q, Bi Y, An J, Lu X, Gao GF*, Wang X*. 2018. Vertical Transmission of the ZikaVirus Causes Neurological Disorders in Mouse Offspring. Sci Rep 8(1):3541.

16.   ZhongS#, Zhang S#, Fan X#, Wu Q#, Yan L#, Dong J, Zhang H, Li L, Sun L, Pan N, Xu X, Tang F*,Zhang J*, Qiao J*, Wang X*. 2018. Asingle-cell RNA-seq survey of the developmental landscape of the humanprefrontal cortex. Nature 555:524-528.

17.   LiuJ#, Liu W#, Yang L#, Wu Q#, Zhang H, Fang A, Li L, Xu X, Sun L, Zhang J*, Tang F*, Wang X*. 2017. The Primate-Specific Gene TMEM14B Marks Outer Radial GliaCells and Promotes Cortical Expansion and Folding. Cell stem cell 21:635-649e638.

所获奖项

1.      项目“大脑皮层发育与相关疾病的分子机制研究”，获北京市人民政府（北京市科学技术奖励工作办公室）颁发的“北京市科学技术奖自然科学奖二等奖”（2021，获奖人排名：3/5），自然科学类省部二等奖。