团队擅长药物的临床前评价，主要是以下方面：

（1）构建神经精神疾病动物模型并用动物模型进行临床前药物筛选

认知损害建模及检测： 空间记忆、 恐惧记忆、 工作记忆、 新异物体识别记忆

脑中风：局灶性短暂及永久性模型

精神分裂症：啮齿类动物构建精分模型

药物成瘾：自身给药、颅内自身电刺激及条件位置偏好

焦虑：高架十字迷宫

抑郁：强迫游泳、悬尾及糖水偏好

脊髓损伤：挫伤及机械损伤

在体行为药理模型以及微透析、颅内显微注射、静脉自身给药、

（2） 离体细胞模型进行药物的临床前评价

氧糖剥夺，离体血脑屏障模型建立，原代神经元培养，原代少突胶质细胞

1.       国家自然科学基金82574397，DDX17介导的胶质淋巴系统功能障碍通过诱导m6A识别蛋白hnRNPL乳酰化引起急性脑缺血后白质损伤的机制

2.       北京市自然科学基金7252192，高同型半胱氨酸通过 PAD4 介导的组蛋白瓜氨酸化阻碍脑缺血恢复期白质修复的机制研究

3.       科技部重点研发项目子课题骨干2022YFC3602805，脑损伤神经移位模型的建立与功能恢复的中枢神经机制研究

4.       国家自然科学基金81870973，GGF2 通过 miR-17～92/Pdlim5/ID2 信号通路促进 NG2 细胞增殖分化改善卒中后神经功能的作用及机制研究

5.       国家自然科学基金，慢性尼古丁暴露调控miR-21/Pdlim5减轻急性脑缺血血脑屏障损伤及其分子机制研究

6.       江苏省自然科学基金面上项目，多巴胺受体偶联的信号通路介导急性脑缺血溶栓期内血脑屏障损伤的机制研究

1. 急性脑梗后如何减少tPA溶栓后的脑出血？

吸烟病人溶栓后出血率比正常人低，从这个现象出发提出尼古丁可减轻脑缺血及tPA引起的血脑屏障损伤

2. 如何促发脑梗后的神经功能恢复？

从白质和血脑屏障完整性出发，刺激内源性干细胞的增值和分化来促进中风后的神经功能恢复

3. 如何减轻精神分裂症引起的认知损害？

精神分裂症病人的吸烟率高，从这个现象提出尼古丁减轻精神分裂症引起的认知损伤

发表论文：

1.       Du W, Geng P, Lian Z, Sun Y, Zhou Y, Guo C, JinX. DPSCs-Exos promote OPCs differentiation and white matter repair viaMyl9-mediated PRMT5 nucleation after ischemic stroke. J Cereb Blood FlowMetab. 2025 Nov 21:271678X251399014. doi: 10.1177/0271678X251399014. Epubahead of print.

2.       Du W, Liu W, Sun Y, Guo C, Geng P, Jin X.Targeting angiogenesis and white matter repair for enhanced functional recoveryafter acute ischemic stroke: A focus on the roles of glial cells. J CerebBlood Flow Metab. 2025 Nov 3:271678X251389346. doi:10.1177/0271678X251389346. Epub ahead of print.

3.       Guo H, Liu W, Sun Y, Jin X, Geng P.Neuroglia and immune cells play different roles in neuroinflammation andneuroimmune response in post-stroke neural injury and repair. Acta PharmacolSin. 2025 Aug 12. doi: 10.1038/s41401-025-01640-5. Epub ahead ofprint. 

4.      Du W, He Y, Li L, Jin X, Geng P. The effects and mechanisms of stemcells and exosomes on neurological function protection and recovery afterischemic stroke. Neuroprotection. 2025; 3: 266-279.

5.       ZhangX#, Du W#, Qian S#, Lu X#,Yu X, Fang H, Dong J, Song M, Sun Y, Wu X, Shen Y, Hao Y, Shen M, Zhou B, WangY*, Xu C*, Jin X*. Glial growth factor 2 treatmentalleviates ischemia and reperfusion-compromised integrity of the blood-brainbarrier through decreasing Mfsd2a/caveolin-1-mediated transcellular andYAP/TAZ-mediated paracellular permeability. Acta Pharmacologica Sinica2024 Q1 IF 7.6

6.       HuX#, Dong J#, Geng P#, SunY#, Du W, Zhao X, Wang Q, Liu C, Wang X, Liu Y, Liu W, ChengH*, Wang W*, Jin X*. Nicotine treatment alleviates acuteischemia stroke-compromised integrity of blood-brain barrier through regulatingPdlim5. Translational Stroke Research. 2024 15,672–687 JCR Q1IF 5.1

7.       WangX#, Wang Q#, Song M#,Wang Y#, Shen X, Sun Y, Guo C, Geng P*, Ma C*, JinX*. Chronic but not acute nicotine treatment ameliorates acuteinflammation-induced working memory impairment by increasing CRTC1 and HCN2 inadult male mice. CNS Neurosci Ther. 2024 Feb;30(2): e14627 JCR Q1  IF 5.9

8.       GaoF, Du W, Guo C, Geng P, Liu W, Jin X*. α7nACh receptor, a promisingtarget to reduce BBB damage by regulating inflammation and autophagy afterischemic stroke. Biomed Pharmacother.2024 Oct;179:117337. Q1IF6.8

9.       WangY#, Liu W#, Zhang J#, Geng P, Jin X**. The role of crosstalk betweencerebral immune cells and peripheral immune cells in the damage and protectionof blood–brain barrier after intracerebral hemorrhage. Brain Hemorrhages,2024,5(3):117-130

10.     WangQ#, Wang M#, Sun Y#, HuX, Geng P, Shu H, Wang X, Wang H, Zhang J, Cheng H, Wang W*, Jin X*.Nicotine pretreatment alleviates MK-801-induced behavioral and cognitivedeficits in mice by regulating Pdlim5/CRTC1 in the PFC. ActaPharmacologica Sinica 2023 44(4):780-790  JCR Q1 IF 7.6

11.     WangY#, Du W#, Hu X, Yu X, Jin X*, WangW*. Targeting blood-brain barrier to delay aging-induced neurologicaldiseases by modulating gut microbe, circadian rhythms and their interplays. ActaPharmaceutica Sinica B 2023 13 (12), 4667-4687 JCR Q1 IF 14.1

12.     WangY#, Du W#, Sun Y#, ZhangJ*, Ma C*, Jin X*. CRTC1 is a potential target to delayaging-induced cognitive deficit by protecting the integrity of the blood-brainbarrier via inhibiting inflammation. Journal of Cerebral Blood Flow andMetabolism 2023;43(7):1042-1059 JCR Q1 IF6.1

13.     HuX#, Geng P#, Zhao X#,Wang Q, Liu C, Guo C, Dong W*, Jin X*. The NG2-glia is apotential target to maintain the integrity of neurovascular unit after acuteischemic stroke. Neurobiology of Disease. 2023 180:106076. JCR Q1IF 5.6

14.     WangY#, Liu W#, Geng P#, DuW, Guo C, Wang Q, Zheng GQ*, Jin X*. Role of crosstalk betweenglial cells and Immune Cells in Blood-Brain Barrier Damage and Protection afterAcute Ischemic Stroke. Aging and Disease, 2023. JCR Q1 IF 7.1

15.     WangQ, Du W, Wang H, et al. Nicotine's effect on cognition, a friend or foe?. ProgNeuropsychopharmacol Biol Psychiatry. 2023;124:110723. Q1 IF 5.2

16.     ChenS#, Sun Y#, Li F#, ZhangX, Hu X, Zhao X, Li Y, Li H, Zhang J, Liu W, Zheng GQ*, Jin X*.Modulation of α7nAchR by melatonin alleviates ischemia andreperfusion-compromised integrity of blood brain barrier through inhibitingHMGB1-mediated microglia activation and CRTC1-mediated neuronal loss. Cellularand Molecular Neurobiology, 2022, 42(7):2407-2422. JCR Q2 IF 4.2

17.     Yan Wang#, Xiaona Wang#, Xinyu Zhang#, Shuang Chen#,YanyunSun, Wenlan Liu, Xinchun JinCA , Guo-qing ZhengCA. D1receptor-mediated endogenous tPA upregulation contributes to blood brainbarrier damage after acute ischemic stroke. Journal of Cellular and MolecularMedicine, 2020, 24(16):9255-66.  IF 5.3（5 Year Impact Factor:5.533）

18.     LiF, Liu WC, Wang Q, Sun Y, Wang H, Jin X. NG2-glia cell proliferation anddifferentiation by glial growth factor 2 (GGF2), a strategy to promotefunctional recovery after ischemic stroke. Biochem Pharmacol.2020;171:113720. Q1 IF 6.8

19.     ShuH#, Wang M#, Song M#, SunY#, Shen X, Zhang J*, Jin X*. Acute nicotinetreatment alleviates LPS-induced impairment of fear memory reconsolidationthrough AMPK activation and CRTC1 upregulation in hippocampus. InternationalJournal of Neuropsychopharmacology. 2020, 23(10):687-699. JCR Q1 IF4.7

20.     WangY#, Wang X#, Zhang X#,Chen S#, Sun Y, Liu W, Jin X*, Zheng G*. D1receptor-mediated endogenous tPA upregulation contributes to blood brainbarrier damage after acute ischemic stroke. Journal of Cellular andMolecular Medicine, 2020, 24(16):9255-66. 4.486 JCR Q2 IF 5

21.     Xinyu Zhang#, Xianzhi Shen#, Jiali Dong#, Wen-Cao Liu#,Yanyun Sun, Hui Shu, Wenlan LiuCA, Chun-feng LiuCA, Xinchun JinCA Inhibitionof reactive astrocytes with fluorocitratealleviates ischemic stroke-induced memory impairment throughupregulating CRTC1 and synaptophysin in rat. Cellular and  MolecularNeurobiology    2019, 39:1151–1163.   IF  3.89（5 Year ImpactFactor:4.780）

22.     Xianzhi Shen, Yanyun Sun, Mengwei Wang, HuiShu, Junfang ZhangCA, Xinchun JinCA. Chronic N-acetylcysteinetreatment alleviates lipopolysaccharide-induced working memory deficit throughupregulating caveolin-1 and synaptophysin in mice.Psychopharmacology 2018, 235(1):179-191 IF 3.54  引用7次（5 Year Impact Factor:4.077）

23.     Yufei Shen, Jingxia Gu, Ziyun Liu, CongyingXu, Shuxia Qian, Xiaoling Zhang, Beiqun Zhou, QiaobingGuan, Yanping WangCA, Xinchun JinCA. Inhibition of HIF-1α reduced bloodbrain barrier damage by regulating MMP-2 and VEGF during acute cerebralischemia. Frontiers in Cellular Neuroscience  2018 12:200  IF  4.3引用7 次2017 年中国脑卒中防治百篇优秀论文“思南奖”（5 Year Impact Factor:5.574）

24.     Svetlana Semenova#, Xinchun Jin#, TristanMcClure-Begley, Matthew Tadman, Michael Marks and AthinaMarkou. Differential effects of intermittent and continuous nicotineexposure on affective and somatic aspects of nicotine withdrawal and expressionof α4β2* nAChRs in rats. Pharmacology Biochemistry Behavior (共同第一作者)  2018,171, 54-65 IF  2.9引用2次

25.     Yanyun Sun, Xi Chen, Xinyu Zhang, Xianzhi Shen, Mengwei Wang,Xiaona Wang, Wen-Cao Liu, Chun-Feng Liu, Jie Liu,Wenlan Liu, XinchunJinCA. ß2-adrenergic receptor mediated HIF-1a upregulationduring acute cerebral ischemia. Frontiers inMolecular Neuroscience  2017, 10:257  IF   5.07 引用16次      获得苏州市自然科学学术论文优秀奖（5 Year Impact Factor:5.760）

26.     Xiaona Wang, Gai-Xiu Xue, Wen-Cao Liu, Hui Shu, Mengwei Wang,Yanyun Sun, Xiaojing Li, Yi Sun, Chun-Feng Liu, Jie LiuCA, Wenlan LiuCA, XinchunJinCA. Melatonin alleviates lipopolysaccharide-compromised integrity ofblood brain barrier by activation of AMP-activated protein kinase (AMPK) inold mice. Aging cell  2017, 16(2):414-421   IF7.6  引用20次（5 Year Impact Factor:9.951）

27.     Yushan Liu, Wencao Liu, Yanyun Sun, Xianzhi Shen, Xiaona Wang,Rong Pan, Hui Shu, Chun-Feng Liu, Wenlan Liu, Ke jian Liu, Xinchun JinCA.Normobaric hyperoxia extends neuro- and vaso-protection of N-Acetylcysteine intransient focal ischemia. MolecularNeurobiology 2017, 54:3418–3427  IF 6.1 引用14 次    2017 年中国脑卒中防治百篇优秀论文“北斗奖” （5 Year Impact Factor:5.512）

28.     Yuan Zhang, Ke Yang, Ting Wang, Weiping Li, Xinchun JinCA,WenlanLiuCA. Nrdp1 increases ischemia induced primary rat cerebral cortical neuronsand pheochromocytoma cells apoptosis via downregulation of HIF-1α protein.Frontiers in Cellular Neuroscience   2017, 11:293  IF 4.55引用3次（5 Year Impact Factor:5.574）

29.     Wen-Cao Liu, Xiaona Wang, Xinyu Zhang, Xi Chen, XinchunJinCA. Melatonin supplementation, a strategy toprevent neurological diseases through maintaining integrity of bloodbrain barrier in old people. Frontiers in Aging Neuroscience 2017,9:165   IF 4.5  引用8次 （5 Year Impact Factor:5.913）

30.     Jie Liu, John Weaver, Xinchun Jin, Yuan Zhang, Ji Xu, KeJ Liu, Weiping Li, Wenlan Liu. The interactions between nitric oxide,caveolin-1 and autophagosome contribute to oxygen-glucose deprivation-inducedof claudin-5 degradation in endothelial cells. MolecularNeurobiology 53(9):5935-5947, 2016  IF 6.1引用32 次（5 Year Impact Factor:5.512）

31.     Xiaona Wang, Yushan Liu, Yanyun Sun,Wenlan Liu, XinchunJinCA. Blood brain barrier breakdown was found in non-infarcted area after2-h MCAO. Journal of Neurological Sciences 2016,363:63-68.    IF 2.44   引用5 次（5 Year Impact Factor:2.661）

32.     Wen-Cao Liu, Xinchun JinCA. Oxygen or cooling, tomake a decision after acute ischemia stroke Medical GasResearch 2016, 6(4):206-211.  引用7 次

33.     Lijun Liang, Jinming Yang, Xinchun JinCA. Cocktailtreatment, a promising strategy to treat acute cerebral ischemicstroke? Medical Gas Research 2016,6(1), 33-38.  引用13 次

34.     Hui Shu, Guo-qing Zheng, Xiaona Wang, Yanyun Sun, Yushan Liu,John Weaver, Xianzhi Shen, Wenlan LiuCA, Xinchun JinCA. Activationof matrix metalloproteinase in dorsal hippocampus drives improvement in spatialworking memory after intra-VTA nicotine infusion in rats. Journal ofNeurochemistry 2015, 135(2):357-367.  IF 4.28引用6 次（5 Year Impact Factor:5.690）

35.     Xinchun JinCA, Yanyun Sun, Ji Xu,Wenlan Liu. Caveolin-1 mediates tissue plasminogen activator-induced MMP-9upregulation in cultured brain microvascular endothelial cells. Journal ofNeurochemistry. 2015,132(6):724-730.   IF 4.28  引用21 次（5 Year Impact Factor:5.690）

36.     Xinchun Jin, Jie Liu and WenlanLiu. Early ischemic blood brain barrier damage: a potential indicator forhemorrhagic transformation following tissue plasminogen activator (tPA)thrombolysis?  Current NeurovascularResearch 2014,11(3):254-262.   IF 2.804 引用32 次（5 Year Impact Factor:2.481）

37.     Xinchun Jin, Jie Liu, Ke J Liu,Gary Rosenberg, Yi Yang, Wenlan Liu. Normobaric hyperoxia combined withminocycline provides greater neuroprotection than either alone in transientfocal cerebral ischemia. ExperimentalNeurology 2013, 240C:9-16.   IF 4.645引40次（5 Year Impact Factor:5.340）

38.     Jie Liu*, Xinchun Jin*, Ke J Liu, Wenlan Liu. Matrixmetalloproteinase-2-mediated occludin degradation and caveolin-1-mediatedclaudin-5 redistribution contribute to blood-brain barrier damage in earlyischemic stroke stage. Journal of Neuroscience 2012, 32(9):3044-57. (共同第一作者)    IF6.908  引用288次（5 Year Impact Factor:2.661）

39.     Xinchun Jin, Jie Liu, Ke J Liu,Yi Yang, Wenlan Liu. Spatiotemporal evolution of BBB damage and tissueinfarction within the first 3 hours after ischemia onset. Neurobiology ofDisease 2012, 48(3):309-316.  IF 5.624引用 41次（5 Year Impact Factor:6.170）

40.     Xinchun Jin, Svetlana Semenova, LiYang, Robert Ardecky, and Athina Markou. The mGluR2 positive allostericmodulator BINA decreases cocaine self-administration and cue-inducedcocaine-seeking and counteracts cocaine-induced enhancement of brain rewardfunction in rats. Neuropsychopharmacology 2010, 35, 2021–2036. (亮点介绍. Neuropsychopharmacology 35,2007–2008).   IF 8.678  引用58次（5 Year ImpactFactor:8.210）

41.     Xinchun Jin, Yufei Lu,Xiaofei Yang, Lan Ma, Baoming Li. Glucocorticoid receptor in thebasolateral nucleus of amygdala is required for post-reactivationreconsolidation of auditory fear memory. European Journal ofNeuroscience 2007, 25, 3702–3712.  IF 3.879  引用74次（5 Year Impact Factor:3.374）

42.     Xinchun Jin, Xuelian Qi,Xiaofei Yang, Baoming Li. Protein synthesis inhibition in the basolateralnucleus of amygdala facilitates extinction of auditory fear memory. ChineseScience Bulletin, 52, 2532-2542, 2007.   IF 1.6（5 Year Impact Factor:1.738）

43.     Xinchun Jin, Chaolin Ma andBaoming Li. The alpha (2A)-adrenoceptor agonist guanfacine improves spatiallearning but not fear conditioning in rats. Acta Physiologica Sinica,59(6):739-744, 2007. 引用11次

专利：

药物组合物及其应用  2016.5.9-2036.5.8

授权号 ZL201610301881.X